Mouse anti-G-CSF, Clone: SPM468, Novus Biologicals
Mouse Monoclonal Antibody
Manufacturer: Novus Biologicals NBP2447970.02MG
Ensure accurate, reproducible results in Flow Cytometry, Immunohistochemistry (Paraffin), ImmunofluorescenceG-CSF Monoclonal antibody specifically detects G-CSF in Human, Monkey samples. It is validated for Flow Cytometry, Immunohistochemistry-Paraffin, Immunofluorescence.
|10mM PBS and 0.05% BSA with 0.05% Sodium Azide|
|Nuclei from pokeweed mitogen stimulated human peripheral blood lymphocytes|
|Cell Cycle and Replication, Cytokine Research|
|Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence|
|Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 1 - 2 ug/ml|
|C17orf33, chromosome 17 open reading frame 33, colony stimulating factor 3 (granulocyte), CSF3OS, filgrastim, G-CSF, GCSFlenograstim, granulocyte colony-stimulating factor, Lenograstim, MGC45931, pluripoietin|
|Protein A or G purified|
|Store at 4C.|
|This MAb recognizes granulocyte-colony stimulating factor (G-CSF) in the cytoplasm of mature granulocytes. It shows no reactivity with any other cell types. Markers of myeloid cells are useful in the identification of different levels of cellular differentiation. It reacts with early precursor and mature forms of myeloid cells. It is useful for the detection of myeloid leukemias and granulocytic sarcomas. It can be used as a marker of granulocytes in normal tissues or inflammatory processes.G-CSF is a pleiotropic cytokine that influences differentiation, proliferation and activation of the neutrophilic granulocyte lineage. The human G-CSF cDNA encodes a 207 amino acid precursor containing a 29 amino acid signal peptide that is proteolytically cleaved to form a 178 amino acid residue mature protein. Two G-CSF s, which are identical except for a three amino acid deletion in the amino-terminus of one form of the protein have been isolated from human cells. Murine and human G-CSF s share 73% sequence identity at the amino acid level.|
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