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Mouse anti-HIF-1 alpha, Clone: HIF1A-84, Azide Free, Novus Biologicals™

Mouse Monoclonal Antibody

Specifications

Antigen HIF-1 alpha
Clone HIF1A-84
Concentration 1.0 mg/mL
Immunogen Recombinant human HIF1 alpha protein
Gene Accession No. Q16665
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Products 2
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
NBP23463501
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Novus Biologicals
NBP2346350.1 MG
0.1 mg This item has been discontinued by the manufacturer and is no longer available. Please call customer service for assistance: 1-800-766-7000. N/A
NBP23463502
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Novus Biologicals
NBP2346350.2 MG
0.2 mg This item has been discontinued by the manufacturer and is no longer available. Please call customer service for assistance: 1-800-766-7000. N/A
Description

Description

HIF-1 alpha Monoclonal antibody specifically detects HIF-1 alpha in Human samples. It is validated for Flow Cytometry, ELISA, Immunocytochemistry/Immunofluorescence, Functional, CyTOF-ready.
Specifications

Specifications

HIF-1 alpha
1.0 mg/mL
Q16665
10mM PBS - BSA free with No Preservative
HIF1 (hypoxia-inducible factor 1), a heterodimeric transcription factor complex central to cellular response to hypoxia, consists of two subunits (HIF-1 alpha and HIF-1 beta) which are basic helix-loop-helix proteins of the PAS (Per, ARNT, Sim) family. Expression of HIF-1 alpha protein is regulated by cellular oxygen level alterations as well as in oxygen-independent manner via different cytokines (through the PI3K-AKT-mTOR pathway), growth factors, oncogenic activation, or loss of tumor suppressor function etc. In normoxic cells, HIF-1 alpha is proline hydroxylated leading to a conformational change that promotes its binding to the VLH (von Hippel Lindau) protein E3 ligase complex; ubiquitination and followed by rapid proteasomal degradation. Hypoxia as well as chemical hydroxylase inhibitors (desferrioxamine, cobalt etc.) inhibit HIF-1 alpha degradation and lead to its accumulation in the cells, whereas, contrastingly, HIF-1 beta/ARNT (AhR nuclear translocator) remains stable under both conditions. Besides their critical role in hypoxic response, HIF1s regulates the transcription of genes responsible for angiogenesis, erythropoiesis/iron-metabolism, glucose metabolism, cell proliferation/survival, adipogenesis, carotid body formation, B lymphocyte development and immune reactions.
Primary
Mouse
Unconjugated
Protein A purified
Flow Cytometry, ELISA, Immunocytochemistry, Immunofluorescence, Functional Assay, CyTOF
HIF1A-84
Recombinant human HIF1 alpha protein
3091
Protein A purified
Monoclonal
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
IgG2b Kappa
Purified
RUO
SDS
Product Certifications

For Research Use Only

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