CD2 Mouse anti-Human, Brilliant Violet 605, Clone: RPA-2.10, BD Optibuild
Mouse Monoclonal Antibody
Manufacturer: BD Biosciences 740391
The RPA-2.10 monoclonal antibody specifically binds to CD2. CD2 is a 50 kDa single-chain transmembrane glycoprotein, also known as LFA-2 or the receptor for sheep erythrocytes. CD2 belongs to the immunoglobulin superfamily of proteins along with its ligand LFA-3, CD58. It is present on about 80-90% of human peripheral blood lymphocytes, greater than 95% of thymocytes, all T lymphocytes that form E-rosettes and a subset of NK cells. CD2 plays a role in T-cell signaling and in lymphocyte adhesion.
This antibody is conjugated to BD Horizon BV605 which is part of the BD Horizon Brilliant Violet family of dyes. With an Ex Max of 407-nm and Em Max of 602-nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20-nm filter set. BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605-nm. Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers. BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser.
|Brilliant Violet 605|
|Aqueous buffered solution containing ≤0.09% sodium azide.|
|Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.|
|LFA-2; Lymphocyte function antigen-2; T11/Leu-5; Rosette receptor|
|Human Phytohemagglutinin-treated Lymphoblasts|
|The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
For Research Use Only