CD45RA Mouse anti-Human, Brilliant Violet 711, Clone: 5H9, BD Optibuild™
Mouse Monoclonal Antibody
Manufacturer: BD Biosciences 740806
The 5H9 monoclonal antibody specifically binds to the human form of the leukocyte common antigen (LCA), CD45RA. This clone also crossreacts with CD45RA expressed by peripheral blood lymphocytes, monocytes and some granulocytes from rhesus macaque, baboon, and cynomolgus monkeys. The observed frequency of CD45RA positive lymphocytes is higher in the non-human primate compared to what is seen with normal human donor lymphocytes.
The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 711-nm. BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors. However, the spillover can be corrected through compensation as with any other dye combination.
|Brilliant Violet 711|
|Aqueous buffered solution containing ≤0.09% sodium azide.|
|The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
|Baboon, Cynomolgus, Human, Rhesus Monkey|
|CD45R; PTPRC; LCA; Leukocyte common antigen|
|Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.|
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