Mouse anti-Kappa Light Chain, Clone: HP6053 + L1C1, Azide Free, Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP2346400.2 MG

Catalog No. NBP23464002

Description

Kappa Light Chain Monoclonal antibody specifically detects Kappa Light Chain in Human samples. It is validated for Western Blot, Flow Cytometry, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, CyTOF-ready.

Specifications

• Antigen: Kappa Light Chain
• Classification: Monoclonal
• Concentration: 1.0 mg/mL
• Dilution: Western Blot 0.5-1.0ug/ml, Flow Cytometry 0.5-2.0ug/million cells, Immunoprecipitation 1-2ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1.0ug/ml, Immunohistochemistry-Frozen 0.5-1.0ug/ml, CyTOF-ready
• Gene Accession No.: P01601
• Gene Symbols: IGKC
• Immunogen: Purified human Ig kappa chain (HP6053) & Human B-Lymphoma Cells (L1C1)
• Purification Method: Protein A purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: This MAb is specific to kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.
• Target Species: Human
• Form: Purified

• Applications: Western Blot, Flow Cytometry, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)
• Clone: HP6053 + L1C1
• Conjugate: Unconjugated
• Formulation: 10mM PBS - BSA free with No Preservative
• Gene Alias: HCAK1, IGKCD, immunoglobulin kappa constant, Km, MGC111575, MGC62011, MGC72072, MGC88770, MGC88771, MGC88809
• Host Species: Mouse
• Molecular Weight of Antigen: 22.5 kDa
• Quantity: 0.2 mg
• Research Discipline: Adaptive Immunity, Immunology
• Gene ID (Entrez): 3514
• Reconstitution: Protein A purified
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Isotype: IgG

For Research Use Only