Mouse anti-Mitochondria, Clone: MTC02, Azide Free, Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP2346860.2 MG

Catalog No. NBP23468602

Description

Mitochondria Monoclonal antibody specifically detects Mitochondria in Human, Mouse (Negative), Rat (Negative) samples. It is validated for Western Blot, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen.

Specifications

• Antigen: Mitochondria
• Classification: Monoclonal
• Concentration: 1.0 mg/mL
• Dilution: Western Blot 0.25-0.5ug/ml, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml, Immunoprecipitation 0.5-1ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1ug/ml
• Host Species: Mouse
• Molecular Weight of Antigen: 60 kDa
• Quantity: 0.2 mg
• Primary or Secondary: Primary
• Reconstitution: Protein A purified
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Isotype: IgG1 κ

• Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
• Clone: MTC02
• Conjugate: Unconjugated
• Formulation: 10mM PBS - BSA free with No Preservative
• Immunogen: Semi-purified mitochondrial preparation
• Purification Method: Protein A purified
• Regulatory Status: RUO
• Test Specificity: This MAb recognizes a 60kDa antigen associated with the mitochondria in human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. This MAb recognizes an antigen associated with the mitochondria in human cells only. It can be used to stain the mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells and may be used to stain mitochondria of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations. This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells.
• Target Species: Human, Mouse (Negative), Rat (Negative)
• Form: Purified

For Research Use Only