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NK-1.1 Mouse anti-Mouse, Brilliant Violet 786, Clone: PK136, BD Optibuild™

Mouse Monoclonal Antibody

Manufacturer:  BD Biosciences 740853

Catalog No. BDB740853


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Description

Description

In the mouse, at least three members of the Klrb ( K iller cell l ectin-like ; formerly NKR-P1 ) gene family have been identified ( Klrb1a/NKR-P1A, Klrb1b/NKR-P1B, and Klrb1c/NKR-P1C ); but in the human gene family, a single homologue has been designated KLRB1, NKR-P1A, or CD161 . The KLRB1/NKR-P1 family of proteins are type-II-transmembrane C-type lectin receptors. KLRB1C/NKR-P1C activates NK-cell cytotoxicity, while KLRB1B/NKR-P1B functions as an inhibitory receptor. KLRB1B/NKR-P1B protein has intracellular Immunoreceptor Tyrosine-based Inhibitory Motif (ITIM), while KLRB1C/NKR-P1C lacks ITIM and activates via association with Fc Receptor γchain. Strikingly, KLRB1B/NKR-P1B and KLRB1C/NKR-P1C share 96% amino acid sequence identity in their extracellular C-type lectin domains. The PK136 antibody reacts with the NK-1.1 surface antigen (CD161c) encoded by the Klrb1c/NKR-P1C gene expressed on natural killer (NK) cells in selected strains of mice (eg, C57BL, FVB/N, NZB, but not A, AKR, BALB/c, CBA/J, C3H, C57BR, C58, DBA/1, DBA/2, NOD, SJL, 129) and the CD161b antigen encoded by the Klrb1b/NKR-P1B gene expressed only on Swiss NIH and SJL mice, but not on C57BL/6. Expression of KLRB1C/NKR-P1C protein is correlated with the ability to lyse tumor cells in vitro and to mediate rejection of bone marrow allografts. The NK-1.1 marker is useful in defining NK cells; however, the antigen is also expressed on a rare, specialized population of T lymphocytes (NK-T cells) and some cultured monocytes. Plate-bound PK136 mAb, in combination with low concentrations of IL-2, induces proliferation of a subset of NK cells.

Specifications

Specifications

NK-1.1
PK136
Brilliant Violet 786
Aqueous buffered solution containing ≤0.09% sodium azide.
Murine
IgG2a, κ
50μg
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Monoclonal
Flow Cytometry
0.2mg/mL
Klrb1b, CD161b, Nkrp1b; Klrb1c, CD161c, NK1.1, Nkrp1c
Affinity Purified
Mouse NK-1+ Spleen and Bone Marrow Cells
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
RUO
Primary
Murine
Documents
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