MUC1 Antibody (139H2), Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP2446660.2MG

Catalog No. NBP24466602

Description

Ensure accurate, reproducible results in ELISA, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

MUC1 Monoclonal antibody specifically detects MUC1 in Human, Mouse samples. It is validated for Flow Cytometry, Immunohistochemistry-Paraffin, Immunofluorescence.

Specifications

• Antigen: MUC-1
• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Dilution: Flow Cytometry 0.25 - 0.5 ug/million cells in 0.1ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 1 - 2 ug/ml
• Gene Accession No.: P15941
• Gene Symbols: MUC1
• Immunogen: Human milk-fat globule membranes (HMFGM)
• Quantity: 0.2 mg
• Research Discipline: Cancer, Cellular Markers, Extracellular Matrix
• Gene ID (Entrez): 4582
• Target Species: Human, Mouse
• Form: Purified

• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
• Clone: 139H2
• Conjugate: Unconjugated
• Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
• Gene Alias: Breast carcinoma-associated antigen DF3, Carcinoma-associated mucin, CD227, CD227 antigen, DF3 antigen, EMA, episialin, H23 antigen, H23AG, KL-6, MAM6, MUC-1, MUC1/ZD, mucin 1, cell surface associated, mucin 1, transmembrane, mucin-1, Peanut-reactive urinary mucin, PEMMUC-1/SEC, PEMT, Polymorphic epithelial mucin, PUMMUC-1/X, tumor associated epithelial mucin, Tumor-associated epithelial membrane antigen, Tumor-associated mucin
• Host Species: Mouse
• Purification Method: Protein A or G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: This MAb reacts with MUC1. The dominant epitope of this MAb has not yet been determined. MUC1 is a large cell surface mucin glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages. It is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and over expressed abundantly in >90% breast carcinomas and metastases. Transgenic MUC1 has been shown to associate with all four c-erbB receptors and localize with c-erbB1 (EGFR) in lactating glands. The MUC1 gene contains seven exons and produces several different alternatively spliced variants. The major expressed form of MUC1 uses all seven exons and is a type 1 transmembrane protein with a large extracellular tandem repeat domain. The tandem repeat domain is highly O glycosylated and alterations in glycosylation have been shown in epithelial cancer cells. Antibody to EMA is useful as a pan-epithelial m
• Content And Storage: Store at 4C.
• Isotype: IgG1 κ

For Research Use Only