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Mouse anti-NF-H, Clone: NE14, Novus Biologicals™

Mouse Monoclonal Antibody

Manufacturer:  Novus BiologicalsSupplier Diversity Partner NBP2447280.1MG

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Catalog No. NBP24472801


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Description

Description

Ensure accurate, reproducible results in Flow Cytometry, Immunohistochemistry (Paraffin)

NF-H Monoclonal antibody specifically detects NF-H in Human, Mouse, Rat, Porcine, Chicken, Feline, Gerbil, Guinea Pig, Primate, Rabbit samples. It is validated for Flow Cytometry, Immunohistochemistry-Paraffin, SDS-Page.
Specifications

Specifications

NF-H
NE14
Unconjugated
10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Purified
NEFH
Crude neurofilament preparation from porcine spinal cord
200 kDa
0.1 mg
Autophagy, Cellular Markers, Cytoskeleton Markers, Neurodegeneration, Neurofilaments, Neuronal Cell Markers, Neuroscience
Primary
4744
Human, Mouse, Rat, Porcine, Chicken, Feline, Gerbil, Guinea Pig, Primate, Rabbit
Flow Cytometry, Immunohistochemistry (Paraffin), SDS-Page
0.2mg/mL
Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.25 - 0.5 ug/ml, SDS-Page
P12036
200 kDa neurofilament protein, KIAA0845, Neurofilament Heavy (200kDa), neurofilament heavy polypeptide, Neurofilament triplet H protein, neurofilament, heavy polypeptide, neurofilament, heavy polypeptide 200kDa, NF200, NFH, NF-H
Mouse
IgG1
Protein A or G purified
RUO
Store at 4C.
Monoclonal
This MAb reacts with a 200kDa protein, identified as heavy sub-unit of neurofilaments (NF-H). It reacts specifically with the phosphorylated KSP/KEP segment at the C-terminus of the heavy subunit (NF-H) of neurofilaments. After dephosphorylation of neurofilaments with alkaline phosphatase, this Ab no longer binds. Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.
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