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Mouse anti-Nucleolin, Clone: 364-5, Novus Biologicals™

Mouse Monoclonal Antibody

Manufacturer:  Novus BiologicalsSupplier Diversity Partner NBP2446120.2MG

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Catalog No. NBP24461202

This item has been discontinued and is no longer available. Please call customer service for assistance: 1-800-766-7000.


Description

Description

Ensure accurate, reproducible results in Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

Specifications

Specifications

Nucleolin
364-5
Unconjugated
10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Purified
NCL
Lysate of SU-DHL-1 Nuclei
76 kDa
0.2 mg
Cell Cycle and Replication, Chromatin Research, Core ESC Like Genes, DNA Repair, DNA replication Transcription Translation and Splicing, Stem Cell Markers
Primary
4691
Human, Bovine (Negative), Mouse (Negative), Rat (Negative)
Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
0.2mg/mL
Western Blot 0.5 - 1.0 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.25 - 0.5 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml
P19338
C23, FLJ45706, FLJ59041, nucleolin, Protein C23
Mouse
IgG1 Kappa
Protein A or G purified
RUO
Store at 4C.
Monoclonal
Recognizes a protein of ~76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.This MAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.
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