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p27/Kip1 Antibody (DCS-72.F6 + KIP1/769), Novus Biologicals™
Mouse Monoclonal Antibody
Supplier: Novus Biologicals NBP2444970.02MG
Description
Ensure accurate, reproducible results in Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
p27/Kip1 Monoclonal specifically detects p27/Kip1 in Human, Mouse, Rat, Monkey samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunofluorescence.Specifications
p27/Kip1 | |
Monoclonal | |
0.2mg/mL | |
Western Blot 0.5 - 1.0 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.25 - 0.5 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml | |
P46527, P46527 | |
CDKN1B | |
Mouse recombinant p27 protein (DCS-72.F6); Recombinant human CDKN1B protein (KIP1/769) | |
Protein A or G purified | |
RUO | |
Primary | |
Recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. Its epitope spans between aa 83-204 of p27. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. This MAb co-precipitates cdk4 in complex p27Kip1 and is excellent for staining of formalin-fixed tissues. | |
Store at 4C. | |
IgG |
Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence | |
DCS-72.F6 + KIP1/769 | |
Unconjugated | |
10mM PBS and 0.05% BSA with 0.05% Sodium Azide | |
CDKN4, cyclin-dependent kinase inhibitor 1B, cyclin-dependent kinase inhibitor 1B (p27, Kip1), Cyclin-dependent kinase inhibitor p27, KIP1P27KIP1, MEN1B, MEN4, p27Kip1 | |
Mouse | |
25.5 kDa | |
0.02 mg | |
Breast Cancer, Cancer, Cell Cycle and Replication, DNA Repair, Prostate Cancer, Tumor Suppressors | |
1027 | |
Human, Mouse, Rat, Monkey | |
Purified |
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