PMEL17/SILV Antibody (HMB45 + PMEL/783), Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP2445230.02MG

Catalog No. NBP24452300

Description

Ensure accurate, reproducible results in Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

PMEL17/SILV Monoclonal specifically detects PMEL17/SILV in Human samples. It is validated for Western Blot, Immunohistochemistry, Immunohistochemistry-Paraffin.

Specifications

• Antigen: PMEL17/SILV
• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Dilution: Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml
• Gene Accession No.: P40967
• Gene Symbols: PMEL
• Immunogen: Extract of pigmented melanoma metastases from lymph nodes (HMB45); Recombinant human SILV protein (PMEL/783)
• Purification Method: Protein A or G purified
• Regulatory Status: RUO
• Gene ID (Entrez): 6490
• Target Species: Human
• Form: Purified

• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
• Clone: HMB45 + PMEL/783
• Conjugate: Unconjugated
• Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
• Gene Alias: D12S53EP1, gp100, ME20, ME20-M, melanocyte protein mel 17, Melanocyte protein Pmel 17, Melanocytes lineage-specific antigen GP100, Melanoma-associated ME20 antigen, melanosomal matrix protein17, PMEL17P100, premelanosome proteinME20M, SI, SIL, silver (mouse homolog) like, silver homolog (mouse), Silver locus protein homolog, silver, mouse, homolog of, SILVPmel17
• Host Species: Mouse
• Molecular Weight of Antigen: 95 kDa
• Quantity: 0.02 mg
• Primary or Secondary: Primary
• Test Specificity: By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This MAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. Metastatic amelanotic melanoma can often be confused with a variety of poorly differentiated carcinomas, large cell lymphomas, and sarcomas using H & E stains alone. It is also difficult to differentiate melanoma from spindle cell carcinomas and various types of mesenchymal neoplasms. This MAb stains fetal and neonatal melanocytes, junctional and blue nevus cells, and malignant melanoma. This MAb also stains Angiomyolipoma (PEComa).
• Content And Storage: Store at 4C.
• Isotype: IgG

For Research Use Only