Mouse anti-Poly-Ubiquitin, Clone: HWA4C4, Novus Biologicals
Mouse Monoclonal Antibody
Manufacturer: Novus Biologicals NBP236523
DescriptionPoly-Ubiquitin Monoclonal antibody specifically detects Poly-Ubiquitin in Human, Non-species specific samples. It is validated for Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.
|Western Blot 1:500 - 1:1000, ELISA 1:100-1:2000, Immunohistochemistry 1:100, Immunocytochemistry/Immunofluorescence 1:100, Immunohistochemistry-Paraffin 1:100|
|Store at -20C. Avoid freeze-thaw cycles.|
|Antibody specifically recognizes K63-linked polyubiquitin chains, but not any other isopeptide-linked (K6, K11, K27, K29, K33, or K48) polyubiquitin chain or monoubiquitin. The specificity of this K63-linked ubiquitin specific monoclonal antibody was demonstrated using synthetic ubiquitin chains, single isopeptide-linked polyubiquitin chains, and with endogenously ubiquitinylated species in both cell lysates and intact cells.|
|ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry (Paraffin), Western Blot|
|PBS with 0.09% Sodium Azide|
|CEP80, HEL112, ribosomal protein S27a, RPS27A, S27A, UBA80, UBC, UBCEP1, UBCEP80|
|The hybridoma secreting the antibody was generated by fusion of splenocytes from Balb/c mice which had received repeated immunisation with a synthetic peptide encompassing the ubiquitin K63-isopeptide motif.|
|Ion exchange chromatography|
|Human, Non-species specific|
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