CD32 Mouse anti-Rat, Brilliant Violet 650, Clone: D34-485, BD Optibuild™
Mouse Monoclonal Antibody
Manufacturer: BD Biosciences 740520
The D34-485 monoclonal antibody specifically recognizes CD32, the Fc γII receptor. Rat CD32 is expressed on B lymphocytes, myeloid cells, and some lymphocytes in the thymic medulla. D34-485 antibody blocks binding of aggregated immunoglobulins to the Fc γII receptors in vitro.
The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm. BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors. However, the spillover can be corrected through compensation as with any other dye combination.
|Brilliant Violet 650|
|Aqueous buffered solution containing ≤0.09% sodium azide.|
|Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.|
|Fcgr2b; FcRII; Fc-gamma RII; FcγRII; FcγII receptor|
|Recombinant Rat CD32 Protein|
|The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
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