Carbonic Anhydrase IX/CA9 Antibody (66.4.C2 (PN-15)), Novus Biologicals™

Mouse Monoclonal Antibody has been used in 5 publications

Supplier:  Novus Biologicals NBP2151990.2MG

Catalog No. NBP21519902

Description

Carbonic Anhydrase IX/CA9 Monoclonal specifically detects Carbonic Anhydrase IX/CA9 in Human, Equine samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.

Specifications

• Antigen: Renal Cell Carcinoma (gp200)
• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Dilution: Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, ELISA 1-5ug/ml for coating, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunoprecipitation 1-2ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1ug/ml
• Gene Accession No.: Q16790
• Gene Symbols: LY75
• Immunogen: Fresh, normal human renal cortical tissue homogenate was used as immunogen to generate the RCC antibody.
• Quantity: 0.2 mg
• Research Discipline: Dendritic Cell Markers, Ovarian Carcinoma Cell Markers, Renal Cell Carcinoma Cell Markers
• Gene ID (Entrez): 4065
• Target Species: Human, Equine
• Form: Purified

• Applications: Western Blot, Flow Cytometry, ELISA, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
• Clone: 66.4.C2 (PN-15)
• Conjugate: Unconjugated
• Formulation: PBS with 0.05% BSA. with 0.05% Sodium Azide
• Gene Alias: CD205LY-75, CLEC13BLy-75, C-type lectin domain family 13 member B, DEC-205CD205 antigen, GP200-MR6, lymphocyte antigen 75
• Host Species: Mouse
• Purification Method: Protein G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Recognizes a glycoprotein of ∼200kDa, identified as carbonic anhydrase IX (CAIX/gp200). Its epitope resides in the carbohydrate domain of gp200. It shows no significant cross-reactivity with other carbohydrate determinants, such as the Lewis blood group antigens, epithelial membrane antigen, HMFG, and AB blood group antigens. In normal kidney, gp200 is localized along the brush border of the pars convoluta and pars recta segments of the proximal tubule, as well as focally along the luminal surface of Bowman's capsule adjoining the outgoing proximal tubule. Reportedly, gp200 is expressed by 93% of primary and 84% of metastatic renal cell carcinomas. This MAb may be useful in the investigations of carcinomas of proximal nephrogenic differentiation especially those showing tubular differentiation.
• Content And Storage: Store at 4C.
• Isotype: IgG2b κ

For Research Use Only