Mouse anti-TAG-72, Clone: B72.3 + CA72/733, Azide Free, Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP2345570.2 MG

Catalog No. NBP23455702

Description

TAG-72 Monoclonal antibody specifically detects TAG-72 in Human, Rat, Bovine, Canine samples. It is validated for Western Blot, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, CyTOF-ready.

Specifications

• Antigen: TAG-72
• Classification: Monoclonal
• Concentration: 1.0 mg/mL
• Dilution: Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunoprecipitation 1-2ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1ug/ml, CyTOF-ready
• Gene Alias: CA 72-4, Protein TAG-72, tag 72, tag-72 Protein TAG-72
• Immunogen: TAG-72 protein (B72.3 & CA72/733)
• Purification Method: Protein A purified
• Regulatory Status: RUO
• Gene ID (Entrez): 182875
• Reconstitution: Protein A purified
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Isotype: IgG

• Applications: Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
• Clone: B72.3 + CA72/733
• Conjugate: Unconjugated
• Formulation: 10mM PBS - BSA free with No Preservative
• Host Species: Mouse
• Molecular Weight of Antigen: 220 kDa
• Quantity: 0.2 mg
• Primary or Secondary: Primary
• Test Specificity: Recognizes an oncofetal antigen of 220kDa, identified as a tumor-associated glycoprotein (TAG-72) with properties of a mucin. This MAb defines the mucin-carried sialylated-Tn epitope. TAG-72 is usually expressed by adenocarcinomas, but is negative in mesotheliomas. Studies have reported that this antibody has 80% sensitivity and 93% specificity for pulmonary adenocarcinoma. Therefore, TAG-72 is a useful marker to distinguish between mesothelioma and adenocarcinoma. However, false positive reactions can occur so results must be interpreted with the utmost caution. This antibody may be useful in the differentiation of non-small cell carcinomas from small cell carcinomas of the lung. The combined use of anti-TAG-72 and anti-GCDFP-15 is valuable in the diagnosis of apocrine carcinoma.
• Target Species: Human, Rat, Bovine, Canine
• Form: Purified

For Research Use Only