Human TNF-alpha Antibody, R&D Systems™

Mouse Monoclonal Antibody has been used in 43 publications

Catalog Number	Mfr. No.	Quantity
MAB610SP	R&D Systems MAB610SP	25 μg
MAB610100	R&D Systems MAB610100	100 μg
MAB610500	R&D Systems MAB610500	500 μg

Description

TNF-alpha Monoclonal specifically detects TNF-alpha in Human samples. It is validated for Western Blot, Neutralization, Immunocytochemistry, ELISA Capture (Matched Antibody Pair).

Specifications

• Antigen: TNF-alpha
• Dilution: Western Blot 1 ug/mL, Neutralization 0.01-0.04 ug/mL, Immunocytochemistry 8-25 ug/mL, ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
• Classification: Monoclonal
• Host Species: Mouse
• Target Species: Human
• Gene Accession No.: P01375
• Gene ID (Entrez): 7124
• Immunogen: E. coli-derived recombinant human TNF-alpha Gly57-Leu233 (predicted) Accession # P01375
• Primary or Secondary: Primary
• Reconstitution: Reconstitute at 0.5 mg/mL in sterile PBS.
• Test Specificity: Detects human TNF-alpha in ELISAs and Western blots. In sandwich ELISAs, less than 0.05% cross-reactivity with recombinant human TNF-beta , recombinant mouse TNF-alpha , recombinant rat TNF-alpha , and recombinant porcine TNF-alpha is observed.

• Clone: 28401
• Applications: Western Blot, Neutralization, Immunocytochemistry, ELISA
• Conjugate: Unconjugated
• Regulatory Status: RUO
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative
• Gene Alias: APC1 protein, Cachectin, Cachetin, DIF, TNF, TNF, monocyte-derived, TNF-a, TNFA, TNFalpha, TNF-alphacachectin, TNFATNF, macrophage-derived, TNFG1F, TNFSF1A, TNFSF2, TNFSF2TNF superfamily, member 2, tumor necrosis factor, tumor necrosis factor (TNF superfamily, member 2), tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2, tumor necrosis factor-alpha
• Gene Symbols: TNF
• Isotype: IgG1
• Purification Method: Protein A or G purified from hybridoma culture supernatant
• Content And Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.