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Mouse anti-ZAP70, Clone: SPM362, Novus Biologicals™

Mouse Monoclonal Antibody

Manufacturer:  Novus BiologicalsSupplier Diversity Partner NBP2445030.1MG

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Catalog No. NBP24450301


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Description

Description

Ensure accurate, reproducible results in Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

ZAP70 Monoclonal antibody specifically detects ZAP70 in Human samples. It is validated for Flow Cytometry, Immunohistochemistry-Paraffin, Immunofluorescence.
Specifications

Specifications

ZAP70
SPM362
Unconjugated
1.0mM PBS and 0.05% BSA with 0.05% Sodium Azide
70 kDa zeta-associated protein, EC 2.7.10, EC 2.7.10.2, SRKFLJ17679, STDFLJ17670, Syk-related tyrosine kinase, tyrosine-protein kinase ZAP-70, TZK, ZAP-70, zeta-chain (TCR) associated protein kinase (70 kD), zeta-chain (TCR) associated protein kinase 70kDa, zeta-chain associated protein kinase, 70kD
Mouse
IgG2a Kappa
Protein A or G purified
RUO
Store at 4C.
Monoclonal
ZAP70 is a 70kDa protein tyrosine kinase found in T-cells and natural killer cells. Control of this protein translation is via the IgVH gene. In Western blotting of whole cell lysates of normal peripheral blood mononuclear cells, the antibody labels a band corresponding to ZAP70. In Western blotting of whole cell lysates of CD19-positive purified leukemia cells from patients with Ig-unmutated and Ig-mutated CLL, the antibody labels a band corresponding to ZAP70 in the Ig-unmutated CLL samples, whereas no band is observed in the Ig-mutated CLL samples. In Western blotting of cell lysates of Jurkat cells (T-lymphoblastic cell line), the antibody labels a band of 70kDa protein. In Western blotting of cell lysates of A431 cells (carcinoma cell line), no band is observed. ZAP70 protein is expressed in leukemic cells of approximately 25% of chronic lymphocytic leukemia (CLL) cases as well. Anti-ZAP70 expression is an excellent surrogate marker for the distinction between the Ig-mutated (anti-ZAP70 negative) and Ig-unmutated (anti-ZAP70 positive) CLL subtypes and can identify patient groups with divergent clinical courses. The anti-ZAP70 positive Ig-unmutated CLL cases have been shown to have a poorer prognosis.
Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
0.2mg/mL
Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml
Purified
ZAP70
Recombinant ZAP-70 protein including residues 1-254 and encompassing SH2 domains of human ZAP70
70 kDa
0.1 mg
Adaptive Immunity, Cell Biology, Immunology, Protein Kinase, Signal Transduction, Tyrosine Kinases
Primary
7535
Human
Documents
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