Mouse anti-ZAP70, Clone: SPM362, Novus Biologicals
Mouse Monoclonal Antibody
Manufacturer: Novus Biologicals NBP2445030.1MG
Ensure accurate, reproducible results in Flow Cytometry, Immunohistochemistry (Paraffin), ImmunofluorescenceZAP70 Monoclonal antibody specifically detects ZAP70 in Human samples. It is validated for Flow Cytometry, Immunohistochemistry-Paraffin, Immunofluorescence.
|1.0mM PBS and 0.05% BSA with 0.05% Sodium Azide|
|70 kDa zeta-associated protein, EC 2.7.10, EC 220.127.116.11, SRKFLJ17679, STDFLJ17670, Syk-related tyrosine kinase, tyrosine-protein kinase ZAP-70, TZK, ZAP-70, zeta-chain (TCR) associated protein kinase (70 kD), zeta-chain (TCR) associated protein kinase 70kDa, zeta-chain associated protein kinase, 70kD|
|Protein A or G purified|
|Store at 4C.|
|ZAP70 is a 70kDa protein tyrosine kinase found in T-cells and natural killer cells. Control of this protein translation is via the IgVH gene. In Western blotting of whole cell lysates of normal peripheral blood mononuclear cells, the antibody labels a band corresponding to ZAP70. In Western blotting of whole cell lysates of CD19-positive purified leukemia cells from patients with Ig-unmutated and Ig-mutated CLL, the antibody labels a band corresponding to ZAP70 in the Ig-unmutated CLL samples, whereas no band is observed in the Ig-mutated CLL samples. In Western blotting of cell lysates of Jurkat cells (T-lymphoblastic cell line), the antibody labels a band of 70kDa protein. In Western blotting of cell lysates of A431 cells (carcinoma cell line), no band is observed. ZAP70 protein is expressed in leukemic cells of approximately 25% of chronic lymphocytic leukemia (CLL) cases as well. Anti-ZAP70 expression is an excellent surrogate marker for the distinction between the Ig-mutated (anti-ZAP70 negative) and Ig-unmutated (anti-ZAP70 positive) CLL subtypes and can identify patient groups with divergent clinical courses. The anti-ZAP70 positive Ig-unmutated CLL cases have been shown to have a poorer prognosis.|
|Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence|
|Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml|
|Recombinant ZAP-70 protein including residues 1-254 and encompassing SH2 domains of human ZAP70|
|Adaptive Immunity, Cell Biology, Immunology, Protein Kinase, Signal Transduction, Tyrosine Kinases|
We continue to work to improve your shopping experience and your feedback regarding this content is very important to us. Please use the form below to provide feedback related to the content on this product.
Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.Ok
For Research Use Only