R&D Systems™ Recombinant Mouse PD-L1/B7-H1 His-tag Protein

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Supplier:  R&D Systems™ 9048B7100

Catalog No. 90-48B-7100

Description

Measured by its binding ability in a functional ELISA. When Recombinant Mouse PD-L1/B7-H1 is immobilized at 25 ng/mL (100 μL/well), the concentration of Recombinant Mouse PD-1 Fc Chimera (Catalog # 1021-PD ) that produces 50% of the optimal binding response is approximately 8-40 ng/mL

Specifications

• Accession Number: Q9EP73
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS.
• Molecular Weight (g/mol): M.W. (Observed): 49-62 kDa, reducing conditions; M.W. (theoretical): 26 kDa
• Source: Mouse myeloma cell line, NS0-derived mouse PD-L1/B7-H1 protein Phe19-His239, with a C-terminal 6-His tag
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Common Name: B7-H1/PD-L1/CD274
• Biological Activity: Measured by its binding ability in a functional ELISA. When Recombinant Mouse B7-H1/PD-L1 is immobilized at 25ng/mL (100μL/well), the concentration of Recombinant Mouse PD-1 Fc Chimera that produces 50% of the optimal binding response is approximately 8-40ng/mL
• Species: Mouse
• Reconstitution: Reconstitute at 400 μg/mL in PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.

• For Use With (Application): Bioactivity
• Gene ID (Entrez): 29126
• Quantity: 100 μg
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Gene Alias: B7-H, B7H1, B7-H1, B7H1PDCD1L1, CD274, CD274 antigenMGC142294, CD274 molecule, PDCD1L1, PDCD1LG1, PDCD1LG1MGC142296, PD-L1, PD-L1B7 homolog 1, PDL1PDCD1 ligand 1, programmed cell death 1 ligand 1, Programmed death ligand 1
• Gene Symbol: Cd274
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Expression System: Mouse myeloma cell line, NS0-derived Phe19-His239, with a C-terminal 6-His tag
• Protein: PD-L1