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R&D Systems™ Mouse Complement Factor D, Carrier-Free Recombinant Protein Available on GSA/VA Contract for Federal Government customers only.

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Manufacturer:  R&D Systems™ 9207SE050

Catalog No. 92-07S-E050

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The Recombinant Mouse Complement Factor D Protein is derived from NS0. The Recombinant Mouse Complement Factor D Protein has been validated for the following applications: Enzyme Activity.

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>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining
<1.0 EU per 1μg of the protein by the LAL method.
Complement Factor D/Adipsin
Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Lys-ThioBenzyl ester (Z-Lys-SBzl), in the presence of 5,5’Dithio-bis (2-nitrobenzoic acid) (DTNB). Edwards, K.M. et al. (1999) J. Biol. Chem. 274:30468. The specific activity is >18 pmol/min/μg, as measured under the described conditions. See Activity Assay Protocol on
Enzyme Activity
Observed M.W.: 40-49kDa, reducing conditions, Theoretical M.W.: 27kDa
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70° C as supplied. 3 months, -20 to -70° C under sterile conditions after opening.
Adipsin, ADN, ADNcomplement factor D, AMBP-1, C3 convertase activator, CFD, complement factor D (adipsin), complement factor D preproprotein, D component of complement (adipsin), DF, EC 3.4.21, EC, PFD, Properdin factor DADIPSIN
Mouse myeloma cell line, NS0-derived Ile26-Ser259, with a C-terminal 10-His tag
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