R&D Systems Mouse Complement Factor D, Carrier-Free Recombinant Protein
Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.
Manufacturer: R&D Systems 9207SE050
DescriptionThe Recombinant Mouse Complement Factor D Protein is derived from NS0. The Recombinant Mouse Complement Factor D Protein has been validated for the following applications: Enzyme Activity.
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|>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining|
|<1.0 EU per 1μg of the protein by the LAL method.|
|Complement Factor D/Adipsin|
|Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Lys-ThioBenzyl ester (Z-Lys-SBzl), in the presence of 5,5’Dithio-bis (2-nitrobenzoic acid) (DTNB). Edwards, K.M. et al. (1999) J. Biol. Chem. 274:30468. The specific activity is >18 pmol/min/μg, as measured under the described conditions. See Activity Assay Protocol on RnDSystems.com.|
|Observed M.W.: 40-49kDa, reducing conditions, Theoretical M.W.: 27kDa|
|Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70° C as supplied. 3 months, -20 to -70° C under sterile conditions after opening.|
|Adipsin, ADN, ADNcomplement factor D, AMBP-1, C3 convertase activator, CFD, complement factor D (adipsin), complement factor D preproprotein, D component of complement (adipsin), DF, EC 3.4.21, EC 220.127.116.11, PFD, Properdin factor DADIPSIN|
|Mouse myeloma cell line, NS0-derived Ile26-Ser259, with a C-terminal 10-His tag|
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