Invitrogen eBioscience Mouse Cytokine Positive Control Cells
Recommended for use as positive control cells when establishing an intracellular cytokine staining protocol.
Manufacturer: Invitrogen 00450051
- Mouse splenocytes were stimulated with ConA (3µg/mL) for two days, followed by recombinant IL-2 (20ng/mL) and IL-4 (20ng/mL) for three days
- The cells were then restimulated with immobilized anti-mouse CD3 (10µg/mL) and soluble anti-mouse CD28 (2µg/mL) in the presence of Brefeldin A for 5 hours
- These cells contain subpopulations that express IL-2, IL-3, IL-4, IL-10, GM-CSF, and IFNγ
- The cells have been fixed with formaldehyde and are provided in a stabilizing buffer so that they may be stored at -80°C for up to six months or at 2 to 8°C for up to one month
|Intracellular Staining Followed by Flow Cytometric Analysis|
|≤−70°C for up to 6 months. Short term storage at 4°C.|
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For Research Use Only