Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Invitrogen™ eBioscience™ Mouse Cytokine Positive Control Cells Non-distribution product as customer accommodation.

Recommended for use as positive control cells when establishing an intracellular cytokine staining protocol.

Manufacturer:  Invitrogen™ 00450051

Catalog No. 50-112-2024


Add to cart

Description

Description

  • Mouse splenocytes were stimulated with ConA (3µg/mL) for two days, followed by recombinant IL-2 (20ng/mL) and IL-4 (20ng/mL) for three days
  • The cells were then restimulated with immobilized anti-mouse CD3 (10µg/mL) and soluble anti-mouse CD28 (2µg/mL) in the presence of Brefeldin A for 5 hours
  • These cells contain subpopulations that express IL-2, IL-3, IL-4, IL-10, GM-CSF, and IFNγ
  • The cells have been fixed with formaldehyde and are provided in a stabilizing buffer so that they may be stored at -80°C for up to six months or at 2 to 8°C for up to one month
Specifications

Specifications

Intracellular Staining Followed by Flow Cytometric Analysis
1mL
≤−70°C for up to 6 months. Short term storage at 4°C.
Documents
Provide Content Correction

The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit

For Research Use Only