R&D Systems Mouse Dkk-2 Recombinant Protein
Induces dose responsive increase in Wntreporter activity in HEK293 cells.
Manufacturer: R&D Systems 2435DKB010/CF
DescriptionMeasured by its ability to inhibit Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells. Recombinant Mouse Wnt-3a (Catalog # 1324-WN ) induces a dose responsive increase in Wnt reporter activity in HEK293 cells. Recombinant Mouse Dkk-2 (Catalog # 2435-DKB/CF) inhibits a constant dose of 100 ng/mL of Recombinant Mouse Wnt-3a (Catalog # 1324-WN). The ED 50 for this effect is 100-600 ng/mL.
Ships at ambient temperature.
|Lyophilized from a 0.2μm filtered solution in PBS|
|Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution.|
|<1.0 EU per 1μg of the protein by the LAL method|
|>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining|
|Spodoptera frugiperda, Sf 21 (baculovirus)-derived Ser26-Ile259, with Leu35Pro substitution and a C-terminal 10-His tag|
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