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Human IL-3 Carrier-Free,Invitrogen™ Non-distribution product as customer accommodation.

Recombinant Protein

Manufacturer:  Invitrogen 34803882

Catalog No. 50-112-3497


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Description

Description

Interleukin 3 (IL-3), also called Mast Cell Growth Factor (MCGF), Multi-Colony Stimulating Factor (multi-CSF), or Eosinophil-CSF (E-CSF), is produced by activated T cells, mast cells and eosinophils. IL-3 is a hematopoietic growth factor that stimulates colony formation of erythroid, megakaryocyte, neutrophil, eosinophil, basophil, mast cell and monocytic lineages. Most of these functions are enhanced or dependent on co-stimulation with other cytokines. Human and mouse IL-3 share only 29% sequence identity at the amino acid level. The bioactivity of IL-3 is highly species specific. For example, human IL-3 has no activity on mouse cells. IL-3 is a potent growth promoting cytokine. IL-3 is capable of supporting the proliferation of a broad range of hematopoietic cell types, and is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. IL-3 has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders. Description: Interleukin 3 (IL-3), also called Mast Cell Growth Factor (MCGF), Multi-Colony Stimulating Factor (multi-CSF), or Eosinophil-CSF (E-CSF), is produced by activated T cells, mast cells and eosinophils. IL-3 is a hematopoietic growth factor that stimulates colony formation of erythroid, megakaryocyte, neutrophil, eosinophil, basophil, mast cell and monocytic lineages. Most of these functions are enhanced or dependent on co-stimulation with other cytokines. Human and mouse IL-3 share only 29% sequence identity at the amino acid level. The bioactivity of IL-3 is highly species specific. Human IL-3 has no activity on mouse cells. Applications Reported: Human IL-3 Recombinant Protein Carrier-Free is biologically active. Applications Tested: The bioactivity of this protein was determined by its ability to induce proliferation of human TF-1 cells. The ED50 for this is less than or equal to 0.1ng/mL, which corresponds to a specific activity of greater than or equal to 1x10^7 Units/mg. Source: E. coli-expressed amino acids Ala20-Phe152 (Accession &Num;NM_000588). Bioactivity: The bioactivity of this protein was determined by its ability to induce proliferation of human TF-1 cells. The ED50 for this is less than or equal to 0.1ng/mL, which corresponds to a specific activity of greater than or equal to 1x10e7 Units/mg. Endotoxin: Less than 0.1ng/ug cytokine as determined by the LAL assay. Purity: >98% as determined by SDS-PAGE and HPLC. Molecular Weight: 15 kDa. Storage and handling: Use in a sterile environment. Store lyophilized protein at less than or equal to -20°C. Reconstituted protein solution can be stored with carrier protein (e.g., 0.1% BSA) at less than or equal to -20°C.
Specifications

Specifications

P08700
Lyophilized
3562
Human IL-3 Carrier-Free
Affinity chromatography
E. coli
RUO
Recombinant
Human IL-3 Carrier-Free
Control, Functional Assay
Recombinant Protein, Carrier-Free
Unlabeled
E. coli
Control, Functional Assay
PBS with no preservative
15kDa
SDS-PAGE and HPLC
100μg
-20°C
E. coli-expressed amino acids Ala20-Phe152 (Accession #NM_000588)
Less than 0.1ng/μg cytokine as determined by the LAL assay
IL3
The bioactivity of this protein was determined by its ability to induce proliferation of human TF-1 cells. The ED50 for this is less than or equal to 0.1ng/mL, which corresponds to a specific activity of greater than or equal to 1x107 Units/mg.
Human
Hu
Recombinant
Safety and Handling

Safety and Handling

ShelfLife : 12 Months

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