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Mouse IFN gamma ELISA Standard,Invitrogen
Manufacturer: Invitrogen 39831165
DescriptionIFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia. Description: Mouse IFN-gamma is a ~20 kDa factor produced by activated T, B and NK cells, and is an anti-viral and anti-parasitic cytokine. IFN-gamma, in synergy with other cytokines such as TNF-alpha, inhibits proliferation of normal and transformed cells. Immunomodulatory effects of IFN-gamma are exerted on a wide range of cell types expressing the high affinity receptors for IFN-gamma. Glycosylation of IFN-gamma does not affect its biological activity. Applications Reported: Recombinant mouse IFN gamma is biologically active. The intended use of the Recombinant Mouse IFN gamma Single-Use ELISA RSG Standard is as a standard for mouse IFN gamma Ready-SET-Go! ELISA and mouse IFN gamma matched antibody pair ELISA. Typically the standard is run in a series of 7 doubling dilutions from the ft.top ft. standard described below. Instructions for use (XMG1.2/R4-6A2 assays): For Ready-SET-Go! ELISA Set (TMB substrate). Dilution: Add 20uL of standard solution to 10 mL of assay diluent to prepare the top standard solution (2000 pg/mL).For matched antibody pair ELISA (ABTS or Super AquaBlue substrate). Dilution: Add 20uL of standard solution to 10 mL of assay diluent to prepare the top standard solution (2000 pg/mL). Instructions for use (AN-18/R4-6A2 assays): For Ready-SET-Go! ELISA Set (TMB substrate). Dilution: Add 20uL of standard solution to 10 mL of assay diluent to prepare 2000 pg/mL solution. Mix thoroughly. Further dilute by adding 50uL of this solution to 0.95 mL of assay diluent to make the top standard solution (100 pg/mL).For matched antibody pair ELISA (ABTS or Super AquaBlue substrate). Dilution: Add 20uL of standard solution to 10 mL of assay diluent to prepare 2000 pg/mL solution. Mix thoroughly. Further dilute by adding 100uL of this solution to 0.90 mLof assay diluent to make the top standard solution (200 pg/mL). Note: These are single-use vials. Use one time and discard. Dilute as suggested above, not difficult-to-measure fractions of the suggested volumes (e.g., not 0.5uL in 1.0 mL) or recovery may not be accurate. Use within 6 hours of dilution to top standard concentration. Applications Tested: This cytokine standard has been calibrated, and subsequently tested by sandwich ELISA using the mouse IFN gamma ELISA pair (XMG1.2/R4-6A2 or AN-18/R4-6A2). These two different assays have different dynamic ranges. Source: E. coli expressed amino acids His 23-Cys 155 of mouse IFN-gamma accession &Num; NM_008337. Purity: >98% as determined by SDS-PAGE. Storage and handling: For best recovery, quick-spin vial prior to opening. For dilution of current stock, use ELISA Assay Diluent.
|E. coli-expressed amino acids His 23-Cys 155 of mouse IFN-gamma accession # NM_008337|
|Mouse IFN gamma ELISA Standard|
|protein with 0.09% sodium azide|
|Mouse IFN gamma ELISA Standard|
Safety and Handling
ShelfLife : 12 Months
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