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F(ab')2-Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 488, Invitrogen™
F(ab')2-Goat Polyclonal Secondary Antibody
Supplier: LIFE TECHNOLOGIES A48286TR
Description
F(ab')2 antibody fragments offer several benefits over whole IgGs, due to their smaller size (∽110 kD vs. ∽ 150 kD) for better penetration into tissue sections, resulting in improved staining in applications such as IHC. The lack of Fc region results in reduced nonspecific background staining when staining cells with Fc receptors (such as immune cells) and other Fc interaction-related interference. F(ab')2 antibody fragments were obtained by a combination of pepsin digestion and immunoaffinity chromatography to remove residual Fc fragments and undigested whole IgG molecules. To minimize cross-reactivity, the goat anti-mouse IgG F(ab')2 fragments have been cross-adsorbed against human, horse and bovine serum proteins to increase specificity of the antibody resulting in higher sensitivity and less background staining. This antibody binds to heavy chains on mouse IgG and light chains on all mouse immunoglobulins, does not bind to human, horse or bovine serum proteins/IgG or mouse non-immunoglobulin serum proteins.
Mouse IgG (H+L) Cross-Adsorbed Polyclonal antibody specifically detects Mouse IgG (H+L) Cross-Adsorbed in Mouse samples. It is validated for Immunocytochemistry, Immunohistochemistry, Western Blot.
Specifications
| Mouse IgG (H+L) Cross-Adsorbed | |
| Polyclonal | |
| Alexa Fluor Plus 488 | |
| Goat | |
| Affinity chromatography | |
| RUO | |
| Mouse | |
| Liquid |
| Immunocytochemistry, Immunohistochemistry, Western Blot | |
| 2 mg/mL | |
| proprietary buffer with 0.016% Bromonitrodioxane, 0.016% Methylisothiazolone; pH 6.5 | |
| Gamma Immunoglobins Heavy and Light chains | |
| 100 μg | |
| Secondary | |
| 4° C, store in dark | |
| IgG |
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