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Invitrogen™ Mouse IgG Isotype Control

Catalog No. 026502
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02-650-2 5 mg
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Catalog No. 02-650-2 Supplier Invitrogen™ Supplier No. 026502
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Mouse Polyclonal Isotype Control

Mouse IgG is purified by various methods from pooled normal mouse serum. The product is pure by SDS-PAGE analysis. The protein concentration is 2.5 mg/mL based on an E (1%) = 14.0 at 280 nm.

The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody's species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.
TRUSTED_SUSTAINABILITY

Specifications

Antigen Mouse IgG
Applications Control
Classification Polyclonal
Concentration 2.5 mg/mL
Conjugate Unconjugated
Formulation PBS with 0.1% sodium azide; pH 7.4
Gene Alias IgG1; Igh-4; Ighg1; Immunoglobulin G; immunoglobulin heavy constant gamma 1 (G1m marker); ImmunoglobulinG; VH7183
Host Species Mouse
Immunogen Gamma Immunoglobin
Purification Method Purified
Quantity 5 mg
Regulatory Status RUO
Primary or Secondary Isotype Control (Primary)
Target Species Not Applicable
Content And Storage -20°C, Avoid Freeze/Thaw Cycles
Product Type Isotype Control
Form Liquid
Isotype IgG
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WARNING: Reproductive Harm - www.P65Warnings.ca.gov
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