Mouse IgG1, Biotin, Isotype control, Novus Biologicals
Mouse Monoclonal Antibody
Manufacturer: Novus Biologicals NBP196888
DescriptionIgG1 Monoclonal antibody specifically detects IgG1 in Mouse samples. It is validated for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Fluorophore-linked immunosorbent assay.
|Lyophilized from 0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free with 0.01% Sodium Azide|
|Protein A purified|
|Reconstitute with 100 ul deionized water|
|Flow Cytometry, Immunocytochemistry, Immunofluorescence|
|Flow Cytometry 1:1000 - 1:5000, Immunocytochemistry/Immunofluorescence 1:1000 - 1:5000, Fluorophore-linked immunosorbent assay 1:100 - 1:2000|
|Store lyophilized antibody at 4°C in the dark. Aliquot reconstituted liquid and store at -20°C. Avoid freeze-thaw cycles.|
|In an Ouchterlony double diffusion assay the material is non-reactive with antisera to mouse IgG2a, IgG2b, IgG3, IgM, and IgA. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG and anti-Mouse serum. Typically, less than 1% cross reactivity was detected by ELISA against other mouse and human heavy or light chains isotypes using chain specific antibodies.|
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