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Mouse IgG1, FITC, clone: ZX3, Isotype Control, Invitrogen™

Catalog No. 01675864
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100 Tests
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Catalog No. Quantity
01-675-864 100 Tests
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Catalog No. 01-675-864 Supplier Thermo Scientific Supplier No. SA112183

Description

Mouse Isotype Control

The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

SA1-12183 has been successfully used in FACS procedures to assess non-specific binding of mouse monoclonal antibodies in human samples.
TRUSTED_SUSTAINABILITY

Specifications

Antigen Mouse IgG1
Applications Control, Flow Cytometry
Clone ZX3
Conjugate FITC
Gene Alias IgG1
Host Species Mouse
Immunogen White blood cells.
Purification Method Protein A/G
Quantity 100 Tests
Regulatory Status RUO
Primary or Secondary Isotype Control (Primary)
Target Species Not Applicable
Content And Storage 4° C, store in dark, DO NOT FREEZE!
Form Liquid
Isotype IgG1 κ
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