Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Super Bright™ 436, eBioscience™, Invitrogen™

Mouse Isotype Control

Catalog Number	Mfr. No.	Quantity
62-471-480	Invitrogen 62471480	25 μg
62-4714-82	Invitrogen 62471482	100 μg

Description

The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

Description: The monoclonal mouse IgG1 K immunoglobulin is useful as an isotype control.Applications Reported: This P3.6.2.8.1 antibody has been reported for use in flow cytometric analysis.Applications Tested: This P3.6.2.8.1 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells and mouse splenocytes. Use the isotype control at the same concentration as the experimental antibody.Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (cat. SB-4400) to minimize any non-specific polymer interactions.Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser

Specifications

• Antigen: Mouse IgG1 kappa
• Concentration: 0.2mg/mL
• Conjugate: Super Bright 436
• Host Species: Mouse
• Target Species: None
• Gene Alias: IgG1, IgG1k
• Primary or Secondary: Isotype Control (Primary)
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!

• Clone: P3.6.2.8.1
• Applications: Flow Cytometry
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
• Isotype: IgG1 κ
• Purification Method: Affinity chromatography

For Research Use Only