Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Super Bright™ 600, eBioscience™, Invitrogen™

Mouse Isotype Control

Supplier:  Invitrogen 63471482

Catalog No. 63-471-482

Description

The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

Mouse IgG1 kappa Control antibody specifically detects Mouse IgG1 kappa in Not Applicable samples. It is validated for Control, Flow Cytometry.

Specifications

• Antigen: Mouse IgG1 kappa
• Clone: P3.6.2.8.1
• Conjugate: Super Bright 600
• Gene Alias: IgG1; Igh-4; Ighg1; Immunoglobulin G; Immunoglobulin G1; immunoglobulin heavy constant gamma 1 (G1m marker); ImmunoglobulinG; ImmunoglobulinG1; VH7183
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Target Species: Not Applicable
• Form: Liquid

• Applications: Control, Flow Cytometry
• Concentration: 0.2 mg/mL
• Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
• Host Species: Mouse
• Quantity: 100 μg
• Primary or Secondary: Isotype Control (Primary)
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Isotype: IgG1 κ

For Research Use Only.