Mouse IgG2a, Biotin, Isotype control, Novus Biologicals
Mouse Monoclonal Antibody
Manufacturer: Novus Biologicals NBP196889
DescriptionIgG2a Monoclonal antibody specifically detects IgG2a in Mouse samples. It is validated for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Fluorophore-linked immunosorbent assay.
|0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2. Stabilizer: 10 mg/mL BSA - Immunoglobulin and Protease free. with 0.01% Sodium Azide|
|Protein A purified|
|Reconstitute with 0.1 ml deionized water (or equivalent) to desired concentration.|
|Flow Cytometry, Immunocytochemistry, Immunofluorescence|
|Flow Cytometry 1:1000 - 1:5000, Immunocytochemistry/Immunofluorescence 1:1000 - 1:5000, Fluorophore-linked immunosorbent assay 1:100 - 1:2000|
|Store lyophilized antibody at 4°C in the dark. Aliquot reconstituted liquid and store at -20°C. Avoid freeze-thaw cycles.|
|In an Ouchterlony double diffusion assay the material is non-reactive with antisera to mouse IgG1, IgG2b, IgG3, IgM, and IgA. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG and anti-Mouse serum. Typically, less than 1% cross reactivity was detected by ELISA against other mouse isotypes using chain specific antibodies.|
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