Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Mouse IgG2a/IgG2b, PE-Cyanine5, Isotype Control, Invitrogen™

Isotype Control Cocktail

Manufacturer:  Invitrogen SA112477

Catalog No. 01-675-870

Add to cart



The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

This antibody contains a mix of IgG2a (Cy5) and IgG2b (RPE) isotypes.SA1-12477 has been successfully used in FACS procedures to assess non-specific binding of mouse monoclonal antibodies in human samples.


Mouse IgG2a/IgG2b
100 Tests
4° C, store in dark, DO NOT FREEZE!, store in dark, DO NOT FREEZE!
Control, Flow Cytometry
PBS with 0.2% carrier protein and 0.08% sodium azide
IgG2, IgG2a, IgG2b
White blood cells.
Protein A/G
Isotype Control
Not Applicable
Provide Content Correction

We continue to work to improve your shopping experience and your feedback regarding this content is very important to us. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit