Mouse IgG2a, FITC, clone: ZX4, Isotype Control, Invitrogen
Mouse Isotype Control
Manufacturer: Invitrogen SA112080
The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.SA1-12080 has been successfully used in FACS procedures to assess non-specific binding of mouse monoclonal antibodies in human samples.
|4° C, do not freeze, do not freeze|
|Control, Flow Cytometry|
|PBS with 0.2% carrier protein and 0.08% sodium azide|
|White blood cells|
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