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Mouse IgG2a kappa Isotype Control (eBM2a), eFluor™ 450, eBioscience™, Invitrogen™
Mouse Isotype Control
Supplier: Invitrogen 48472482
Description
The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.
Description: This is a monoclonal mouse IgG2a, kappa antibody. It is used as an isotype control for mouse IgG2a antibodies.Applications Reported: Mouse IgG2a K Isotype Control eFluor™ 450 has been reported for use in flow cytometric analysis.Applications Tested: eFluor™ 450 Mouse IgG2a, K Isotype Control has been tested by flow cytometric analysis of normal human peripheral blood cells. Use isotype control at the same concentration as experimental antibody.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2μm post-manufacturing filtered.Specifications
| Mouse IgG2a kappa | |
| eBM2a | |
| eFluor 450 | |
| IgG2, IgG2a, IgG2ak | |
| Affinity chromatography | |
| RUO | |
| Not Applicable | |
| Liquid |
| Control, Flow Cytometry | |
| 0.2mg/mL | |
| PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2 | |
| Mouse | |
| 100 μg | |
| Control | |
| 4° C, store in dark, DO NOT FREEZE! | |
| IgG2a κ |
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For Research Use Only.