Mouse IgG2a kappa Isotype Control (eBM2a), Functional Grade, eBioscience™, Invitrogen™

Mouse Isotype Control

Catalog Number	Mfr. No.	Quantity
50-145-35	Invitrogen 16472481	50 μg
50-145-36	Invitrogen 16472482	100 μg
50-145-37	Invitrogen 16472485	500 μg

Description

Description: This is a monoclonal mouse IgG2a, kappa antibody. It is used as an isotype control for mouse IgG2a antibodies. Applications Reported: This mouse IgG2a isotype control has been reported for use in bioassays and cell surface and intracellular flow cytometric analysis. Applications Tested: This mouse IgG2a isotype control has been tested by flow cytometric analysis of normal human peripheral blood cells. It should be used at the same concentration as the experimental antibody. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Storage and handling: Use in a sterile environment. Filtration: 0.2 μm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC.

Mouse IgG2a is a subtype of immunoglobulin G antibody that is produced by B cells in response to an antigen. It is the second most abundant IgG subtype in the serum and plays a crucial role in the adaptive immune response. Mouse IgG2a is composed of two heavy chains and two light chains, and it is involved in opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity. It is also involved in the regulation of immune responses, including the activation of T cells and the inhibition of B cell activation. Mouse IgG2a is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry. It is also used in therapeutic applications, such as immunotherapy and vaccine development.

Specifications

• Antigen: Mouse IgG2a kappa
• Concentration: 1 mg/mL
• Conjugate: Functional Grade
• Host Species: Mouse
• Target Species: Not Applicable
• Gene Alias: IgG2; Immunoglobulin G; Immunoglobulin G2; ImmunoglobulinG; ImmunoglobulinG2
• Primary or Secondary: Isotype Control (Primary)
• Content And Storage: 4° C

• Clone: eBM2a
• Applications: Control, Flow Cytometry, Functional Assay
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with no preservative; pH 7.2
• Isotype: IgG2a κ
• Purification Method: Affinity chromatography

For Research Use Only.