Invitrogen™ Mouse IgG2a kappa Isotype Control (eBM2a), PE-Cyanine7, eBioscience™

Catalog No.	Quantity
50-155-70	50 μg

Catalog No. 50-155-70 Supplier Invitrogen™ Supplier No. 25472481

Description

Mouse Monoclonal Isotype Control

This is a monoclonal mouse IgG2a antibody. It is used as an isotype control for mouse IgG2a antibodies. Applications Reported: This eBM2a antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBM2a antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. Use isotype control at the same concentration as experimental antibody. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered.

Mouse IgG2a is a subtype of immunoglobulin G antibody that is produced by B cells in response to an antigen. It is the second most abundant IgG subtype in the serum and plays a crucial role in the adaptive immune response. Mouse IgG2a is composed of two heavy chains and two light chains, and it is involved in opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity. It is also involved in the regulation of immune responses, including the activation of T cells and the inhibition of B cell activation. Mouse IgG2a is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry. It is also used in therapeutic applications, such as immunotherapy and vaccine development.

Specifications

• Antigen: Mouse IgG2a kappa
• Applications: Control, Flow Cytometry
• Classification: Monoclonal
• Clone: eBM2a
• Concentration: 0.2 mg/mL
• Conjugate: PE-Cyanine7
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Alias: IgG2; Immunoglobulin G; Immunoglobulin G2; ImmunoglobulinG; ImmunoglobulinG2
• Host Species: Mouse
• Purification Method: Affinity chromatography
• Quantity: 50 μg
• Regulatory Status: RUO
• Primary or Secondary: Isotype Control (Primary)
• Target Species: Not Applicable
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Product Type: Isotype Control
• Form: Liquid
• Isotype: IgG2a κ