Mouse IgG2a kappa Isotype Control (eBM2a), Super Bright™ 645, eBioscience™, Invitrogen™

Mouse Isotype Control

Catalog Number	Mfr. No.	Quantity
64472482	Invitrogen 64472482	100 μg

Description

The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

Mouse IgG2a kappa Control antibody specifically detects Mouse IgG2a kappa in Not Applicable samples. It is validated for Control, Flow Cytometry.

Specifications

• Antigen: Mouse IgG2a kappa
• Conjugate: Super Bright 645
• Host Species: Mouse
• Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
• Primary or Secondary: Isotype Control (Primary)

• Clone: eBM2a
• Form: Liquid
• Regulatory Status: RUO
• Isotype: IgG2a κ
• Purification Method: Affinity chromatography

For Research Use Only