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Invitrogen™ eBioscience™ Mouse IL-1 alpha ELISA Ready-SET-Go!™ Kit Non-distribution product as customer accommodation.

Specifically engineered for accurate and precise measurement of protein levels from samples including serum, cell lysates, and cell culture supernatants. Interleukin- 1 alpha (IL-1 alpha) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells.

Manufacturer:  Invitrogen™ 88501922

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Catalog No. 50-112-5107


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Includes: Capture Antibody: Pre-titrated, purified antibody
Detection Antibody. Pre-titrated, biotin-conjugated antibody
Standard. Recombinant protein for generating standard curve and calibrating samples
Coating Buffer. 10X PBS ELISA Coating Buffer
Assay Diluent. 5X Concentrated
Detection Enzyme. Pre-titrated Avidin-HRP
Substrate Solution. Tetramethylbenzidine (TMB) Solution
Certificate of Analysis. Lot-specific instructions for dilution of antibodies and standards.

Description

Description

IL-1 alpha (Interleukin-1 alpha) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 alpha is coded by the IL1A gene and signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 beta. IL1A is located on the q arm on chromosome 2 at position 13. IL-1 alpha plays an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses. IL-1 alpha activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 alpha is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. Further, IL-1 alpha is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury and induces apoptosis. The IL1A gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. IL1 alpha regulates the activities of NF-kappaB and mitogen-activated protein kinases. IL-1 alpha can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of IL-1 alpha are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.
Specifications

Specifications

P01582
Biotin
16175
Colorimetric Microplate Reader
ELISA Kit
RUO
Plasma,Serum,Supernatant
Mouse
1 hr 45 min
Mouse
Interleukin-1 alpha, IL1F1, Hematopoietin-1
4-500 pg/mL
ELISA
96-well plates
96-well Plates: Corning Costar flat-bottom plates (included with Cat. Nos. ending in -22; -76; and -86)
2 x 96 tests
4 pg/mL
Plasma,100 μL|Serum,100 μL|Supernatant,100 μL
2-8°C
24 hr 30 min
Il1a
HRP
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