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Invitrogen™ eBioscience™ Mouse IL-1 beta ELISA Ready-SET-Go!™ Kit Non-distribution product as customer accommodation.

Specifically engineered for accurate and precise measurement of mouse IL-1 beta protein levels from samples including serum, protein lysate and cell culture supernatants. This ELISA set will recognize the cleaved, mature form and uncleaved pro-form of Mouse IL-1 beta.

Manufacturer:  Invitrogen™ 88701386

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Catalog No. 50-112-8807


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Includes: Capture Antibody: Pre-titrated, purified antibody
Detection Antibody. Pre-titrated, biotin-conjugated antibody
Standard. Recombinant protein for generating standard curve and calibrating samples
10X Coating Buffer . Buffer for plating the Capture Antibody
5X ELISA/ELISPOT Diluent . Buffer for blocking and diluting the Detection Antibody and Enzyme
Enzyme. Pre-titrated Avidin-HRP
Substrate. 1X TMB Solution

Description

Description

Interleukin-1 beta (IL-1 beta) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 beta is synthesized in response to inflammatory stimuli as a 31 kDa inactive pro-form that accumulates in the cytosol. Cleavage of pro-IL-1 beta into the active 17 kDa protein requires the activation of inflammasomes, which are multi-protein complexes that respond to pathogens, stress conditions, and other danger signals. Inflammasome activation triggers the processing of the caspase-1 precursor into its active form, which in turn cleaves pro-IL-1 beta. IL-1 beta lacks a signal sequence peptide for classical ER/Golgi pathway and is secreted alongside caspase-1 via an alternate and incompletely understood mechanism. Although IL-1 beta is most often secreted in its active form, secretion of the uncleaved protein may be detectable under some biological conditions. IL-1 beta signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 alpha. IL-1 beta activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 beta play an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses to infection and injury. Elevated levels of IL-1 beta have been associated with many chronic inflammatory conditions IL-1 beta neutralizing antibodies potential therapeutic value.
Specifications

Specifications

P10749
Biotin
16176
Colorimetric Microplate Reader
ELISA Kit
RUO
Cell Lysate,Serum,Supernatant
Mouse
1 hr 45 min
Mouse
Interleukin-1 beta
8-1,000 pg/mL
ELISA
96-well plates
10X Coating Buffer: Buffer for plating the Capture Antibody
10 x 96 tests
8 pg/mL
Cell Lysate,100 μL|Serum,100 μL|Supernatant,100 μL
2-8°C
24 hr 30 min
Il1b
HRP
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