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Human Wnt-7a Carrier-Free,Invitrogen™ Non-distribution product as customer accommodation.

Recombinant Protein

Manufacturer:  Invitrogen 348694166

Catalog No. 50-112-3516


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Description

Description

Wnt proteins constitute a family of secreted glyocoproteins that activate signal transduction pathways to mediate tissue homeostasis, cell fate, cell proliferation, and self-renewal. The canonical signaling pathway is initiated upon Wnt binding to a Frizzled family receptor and an LRP5/LRP6 coreceptor, which leads to activation of the beta-catenin cascade. Non-canonical Wnt signals are activated by binding to a Frizzled family receptor and ROR2 coreceptor. Extensive research has shown that deregulation of these pathways or its components can lead to cancer. Human Wnt-1 is encoded as a 370 amino acid precursor that, upon secretion, is cleaved into a 343 amino acid (42 kDa) mature form. At the amino acid level, mature human Wnt-1 is approximately 99% identical to mature mouse Wnt-1. Depending on the tissue, Wnt-1 can activate the canonical and non-canonical signaling pathways. Studies have demonstrated its involvement in a variety of functions, including embryonic development and carcinogenesis. Wnt-1 signaling can also induce the phosphorylation and subsequent degradation of c-Myb. Description: Wnt proteins constitute a family of secreted glyocoproteins that activate signal transduction pathways to mediate tissue homeostasis, cell fate, cell proliferation, and self-renewal. The canonical signaling pathway is initiated upon Wnt binding to a Frizzled family receptor and an LRP5/LRP6 coreceptor, which leads to activation of the beta-catenin cascade. Non-canonical Wnt signals are activated by binding to a Frizzled family receptor and ROR2 coreceptor. Extensive research has shown that deregulation of these pathways or its components can lead to cancer. Human Wnt-7a is encoded as a 349 amino acid precursor that, upon secretion, is cleaved into a 318 amino acid (48 kDa) mature form. At the amino acid level, the mature form of human Wnt-7a is approximately 98% identical to mature mouse Wnt-7a. Depending on the tissue, Wnt-7a can activate the canonical and non-canonical signaling pathways. Studies have demonstrated its involvement in a variety of functions, including neurogenesis, muscle regeneration, and carcinogenesis. Applications Reported: Recombinant human Wnt-7a is biologically active and can inhibit Wnt-3a-induced alkaline phosphatase production in MC3T3-E1 cells. Applications Tested: The ED50 of this protein, as determined by Wnt-3a-induced alkaline phosphatase production in MC3T3-E1 cells, is 40-60ng/mL. Source: HEK293 cells (amino acids Leu32-Lys349; accession &Num; NP_004616.2). Bioactivity: The ED50 of this protein, as determined by inhibition of Wnt-3a-induced alkaline phosphatase production in MC3T3-E1 cells, is 40-60ng/mL. Endotoxin: Less than 0.1ng/ug cytokine as determined by the LAL assay. Purity: >80% as demonstrated by SDS-gel electrophoresis and reverse phase HPLC. Molecular Weight: 48 kDa (mature form). Storage and handling: For best recovery, quick spin vial prior to opening. Reconstitute in water to a concentration of less than or equal to 0.2mg/mL. Do not vortex. Use in sterile environment. Store lyophilized protein at less than or equal to -20°C. Reconstituted protein solution can be stored with carrier-protein (e.g., 0.1% BSA) at less than or equal to -20°C.
Specifications

Specifications

O00755
Lyophilized
7476
Human Wnt-7a Carrier-Free
SDS-PAGE and HPLC
15μg
-20°C
HEK293 cells (amino acids Leu32-Lys349; Accession # NP_004616.2)
Less than 0.1ng/μg cytokine as determined by the LAL assay
WNT7A
The ED50 of this protein, as determined by inhibition of Wnt-3a-induced alkaline phosphatase production in MC3T3-E1 cells, is 40-60ng/mL.
Human
Hu
Recombinant
Control, Functional Assay, Neutralization
PBS with 0.5% CHAPS and no preservative; pH 7.5
48kDa (mature form)
7.5
Affinity chromatography
HEK 293 cells
RUO
Recombinant
Human Wnt-7a Carrier-Free
Control, Functional Assay, Neutralization
Recombinant Protein, Carrier-Free
Unlabeled
HEK293 cells
Safety and Handling

Safety and Handling

ShelfLife : 12 Months

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