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Invitrogen™ eBioscience™ Mouse IL-12/IL-23 total p40 ELISA Ready-SET-Go!™ Kit Non-distribution product as customer accommodation.

Specifically engineered for accurate and precise measurement of mouse IL-12 / IL-23 (total p40) protein levels from samples including serum, and cell culture supernatants.

Manufacturer:  Invitrogen™ 88712088

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Catalog No. 50-112-9005


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Alert:

Contains Azide


Includes: Capture Antibody: Pre-titrated, purified antibody
Detection Antibody. Pre-titrated, biotin-conjugated antibody
Standard. Recombinant cytokine for generating standard curve and calibrating samples
ELISA/ELISPOT Coating Buffer Powder. This Ready-Set-Go! ELISA Set may contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 uM)) or 10X PBS ELISA Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20).
Assay Diluent. 5X concentrated
Detection enzyme. Pre-titrated Avidin-HRP
Substrate Solution. Tetramethylbenzidine (TMB) Substrate Solution

Description

Description

IL-12 is an interleukin produced by dendritic cells, neutrophils, macrophages and human lymphoblastoid cells as a result of antigenic stimulation. IL-12 is a heterodimeric cytokine encoded by IL-12A (p35) and IL-12B (p40). IL-23 is a heterodimeric cytokine that shares a subunit with IL-12 (IL-12B) along with an IL-23A (IL-23p19) subunit. Functionally, IL-12 increases the expression of vascular endothelial adhesion molecule-1 on the endothelium, directs leukocytes towards tumors and facilitates the ischemic-hemorrhagic necrosis of the tumor tissue. In comparison, IL-23 plays an important role in stimulating memory T-cells, and is required for the induction, expansion, maintenance and downstream effector functions of Th17 cells, which play a vital role in upregulating neutrophil chemokines and various pro-inflammatory cytokines.
Specifications

Specifications

P43432,Q9EQ14
Biotin
16160, 83430
96 Well Plate: Corning Costar 9018 (included with product Cat. Nos. ending in suffixes -22; -44; -76; -86)
10 x 96 tests
2 pg/mL
Serum,100 μL|Supernatant,100 μL
2-8°C
24 hr 30 min
Il12b, Il23a
HRP
2-300 pg/mL
ELISA
Colorimetric Microplate Reader
ELISA Kit
RUO
Serum,Supernatant
Mouse
1 hr 45 min
Mouse
Interleukin-12, IL12, IL23
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