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Invitrogen™ eBioscience™ Mouse IL-17AF (heterodimer) ELISA Ready-SET-Go!™ Kit, 2nd Generation Non-distribution product as customer accommodation.

Specifically engineered for accurate and precise measurement protein levels from samples including serum and cell culture supernatants.

Manufacturer:  Invitrogen™ 88871122

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Catalog No. 50-112-5226


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Alert:

Contains Sodium azide


Includes: Capture Antibody: Pre-titrated, purified antibody
Detection Antibody. Pre-titrated, biotin-conjugated antibody
Standard. Recombinant cytokine for generating standard curve and calibrating samples
Coating Buffer 10X PBS ELISA Coating Buffer.
Assay Diluent. 5X concentrated
Sample Diluent A
Detection enzyme. Pre-titrated Avidin-HRP
Substrate Solution. Tetramethylbenzidine (TMB) Substrate Solution

Description

Description

Interleukin-17A (IL-17A) is a CD4+ T cell-derived cytokine that promotes inflammatory responses in cell lines and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection. The cDNA encoding human IL-17A was isolated from a library of CD4+ T cells; the encoded protein exhibits 72 percent amino acid identity with HVS13 , an open reading frame from a T lymphotropic Herpesvirus saimiri, and 63 percent with mouse CTLA-8 (cytotoxic T-lymphocyte associated antigen-8). Human IL-17A exists as glycosylated 20-30 kD homodimers. High levels of IL-17A homodimer are produced by activated peripheral blood CD4+ T-cells. IL-17A enhances expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts. Human IL-17A also stimulates epithelial, endothelial, or fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2. In the presence of human IL-17A, fibroblasts can sustain the proliferation of CD34+ hematopoietic progenitors and induce maturation into neutrophils. Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating that all homologs can recognize the mouse receptor. IL-17A binds to a receptor that binds also to HVS13 (viral IL-17) and to CTLA-8.This recombinant protein is E. coli expressed. Insect cell expressed Human IL-17A Recombinant Protein (cat. 14-8180) is also available.
Specifications

Specifications

Q62386,Q7TNI7
Biotin
16171, 257630
Colorimetric Microplate Reader
ELISA Kit
RUO
Serum,Supernatant
Mouse
1 hr 45 min
Mouse
Interleukin-17A/F
16-2,000 pg/mL
ELISA
96-well plates
96 Well Plate: Corning Costar 9018 (included with product Cat. Nos. ending in suffixes -22; -44; -76; -86)
2 x 96 tests
16 pg/mL
Serum,50 μL|Supernatant,50 μL
2-8°C
24 hr 30 min
Il17a, Il17f
HRP
Documents
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