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Invitrogen™ IL-5 Mouse Uncoated ELISA Kit Non-distribution product as customer accommodation.

Uncoated ELISA Kit

Manufacturer:  Invitrogen™ 88705477

Catalog No. 50-172-10


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Alert:

Contains Azide


Includes: Capture Antibody: Pre-titrated, purified antibody
Detection Antibody. Pre-titrated, biotin-conjugated antibody
Standard. Recombinant protein for generating standard curve and calibrating samples
10X Coating Buffer . Buffer for plating the Capture Antibody
5X ELISA/ELISPOT Diluent . Buffer for blocking and diluting the Detection Antibody and Enzyme
Enzyme. Pre-titrated Avidin-HRP
Substrate. 1X TMB Solution

Description

Description

The Mouse Interleukin-5 (IL-5) Uncoated ELISA Kit contains pre-matched antibody pairs, and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of mouse IL-5. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

IL-5 is a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. IL-5 cytokine is a main regulator of eosinopoiesis, eosinophil maturation and activation. The elevated production of this cytokine is reported to be related to asthma or hypereosinophilic syndromes. The receptor of IL-5 cytokine is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). IL-5, together with those for interleukin 4 (IL4), interleukin 13 (IL13), and CSF2, form a cytokine gene cluster on chromosome 5. IL-5, IL4, and IL13 are found to be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31.
Specifications

Specifications

P04401
Lavage Fluid, Serum, Supernatant
4-500 pg/mL
ELISA
Colorimetric Microplate Reader
ELISA Kit
4 pg/mL
2°C to 8°C
24 hr. 30 min.
Il5
HRP
20 x 96 Tests
Mouse
Biotin
16191
10X Coating Buffer: Buffer for plating the Capture Antibody
RUO
Lavage Fluid,100 μL|Serum, 100 μL|Supernatant, 100 μL
1 hr. 45 min.
Mouse
Interleukin-5
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