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Invitrogen™ eBioscience™ Mouse IL-6 ELISA Ready-SET-Go!™ Kit Non-distribution product as customer accommodation.

Specifically engineered for accurate and precise measurement of mouse IL-6 protein levels from samples including serum, plasma, cell lysates and cell culture supernatants.

Manufacturer:  Invitrogen™ 88706488

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Catalog No. 50-112-8863


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Alert:

Contains Azide


Includes: Capture Antibody: Pre-titrated, purified antibody
Detection Antibody. Pre-titrated, biotin-conjugated antibody
Standard. Recombinant protein for generating standard curve and calibrating samples
10X Coating Buffer . Buffer for plating the Capture Antibody
5X ELISA/ELISPOT Diluent . Buffer for blocking and diluting the Detection Antibody and Enzyme
Enzyme. Pre-titrated Avidin-HRP
Substrate. 1X TMB Solution

Description

Description

Interleukin 6 (IL-6) is a multifunctional 26 kD protein originally discovered in the medium of RNA-stimulated fibroblastoid cells. IL-6 appears to be directly involved in the responses that occur after infection and cellular injury, and it may prove to be important as IL-1 and TNF-a in regulating the acute phase response. IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages and endothelial cells. IL-6 acts upon a variety of cells including myeloid progenitor cells, T cells, B cells, and hepatocytes. In addition, IL-6 appears to interact with IL-2 in the proliferation of T lymphocytes. IL-6 potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors. IL-6 plays a critical role in B-cell differentiation to plasma cells and is a potent growth factor for plasmacytoma and myeloma. IL-6 is a very useful culture supplement for the generation of a high number of antibody-producing hybridomas. Primarily produced at sites of acute and chronic inflammation, IL-6 is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of IL-6 is implicated in a wide variety of inflammation-associated disease states including diabetes mellitus and systemic juvenile rheumatoid arthritis.
Specifications

Specifications

P08505
Biotin
16193
10X Coating Buffer: Buffer for plating the Capture Antibody
10 x 96 tests
4 pg/mL
Cell Lysate,100 μL|Plasma,100 μL|Serum,100 μL|Supernatant,100 μL
2-8°C
24 hr 30 min
Il6
HRP
4-500 pg/mL
ELISA
Colorimetric Microplate Reader
ELISA Kit
RUO
Cell Lysate,Plasma,Serum,Supernatant
Mouse
1 hr 45 min
Mouse
Interleukin-6
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