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Mouse LIF, Recombinant Protein, eBioscience™ Non-distribution product as customer accommodation.

Recombinant Protein

Manufacturer:  Invitrogen 14852180

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Catalog No. 50-112-8870


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Description

Description

Leukemia inhibitory factor (LIF) is a 20 kDa protein that belongs to the IL-6 receptor family. It binds to a heterodimeric membrane receptor made up of a LIF-specific subunit, gp190 or LIFR, and the subunit gp130, which is shared with the other members of the IL-6 family. LIF expression has been observed in various tissues including thymus, lung, and neuronal tissue. LIF can be up-regulated by pro-inflammatory cytokines such as TNFα and IL-17, and elevated levels of LIF have been found in cases of rheumatoid arthritis, neural injury, systemic inflammation, and tuberculosis. LIF displays diverse biological effects, but is best known for its ability to inhibit the differentiation of embryonic stem cells in mice and contribute to stem cell self-renewal.Human and mouse LIF share 79% sequence homology and exhibit cross-species activity. However, LIF inhibition of stem cell differentiation appears to be mouse-specific. Description: Leukemia inhibitory factor (LIF) is a 20 kDa protein that belongs to the IL-6 receptor family. It binds to a heterodimeric membrane receptor made up of a LIF-specific subunit, gp190 or LIFR, and the subunit gp130, which is shared with the other members of the IL-6 family. LIF expression has been observed in various tissues including thymus, lung, and neuronal tissue. LIF can be up-regulated by pro-inflammatory cytokines such as TNFalpha and IL-17, and elevated levels of LIF have been found in cases of rheumatoid arthritis, neural injury, systemic inflammation, and tuberculosis. LIF displays diverse biological effects, but is best known for its ability to inhibit the differentiation of embryonic stem cells in mice and contribute to stem cell self-renewal.Human and mouse LIF share 79% sequence homology and exhibit cross-species activity. However, LIF inhibition of stem cell differentiation appears to be mouse-specific. Applications Reported: Recombinant mouse LIF is biologically active. Applications Tested: Recombinant mouse LIF has been tested for induction of mIL-6 in the M1 cell line. The ED50 in this assay is 0.75-0.4ng/mL, which corresponds to a specific activity of 1.3 x 10e6 - 2.5 x 10e6 Units/mg. Source: E. coli derived Ser24-Phe203 accession number NM_008501. Bioactivity: Recombinant mouse LIF has been tested for induction of mIL-6 in the M1 cell line. The ED50 in this assay is 0.75-0.4ng/mL, which corresponds to a specific activity of 1.3 x 10e6 - 2.5 x 10e6 Units/mg. When used at 10ng/mL, this recombinant protein has also been found to support mouse embryonic stem cells in an undifferentiated state at same concentration. Endotoxin: Less than 0.01ng/ug cytokine as determined by the LAL assay. Purity: >98% as determined by SDS-PAGE. Molecular Weight: 20 kDa. Storage and handling: For best recovery, quick-spin vial prior to opening. Use in a sterile environment. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2µm post-manufacturing filtered.
Specifications

Specifications

P09056
Control, Functional Assay
PBS with 0.05% Tween 20, 1% BSA and no preservative
20kDa
SDS-PAGE
25μg
-80°C
E. coli derived Ser24-Phe203 accession number NM_008501
Less than 0.01ng/μg cytokine as determined by the LAL assay
Lif
Recombinant mouse LIF has been tested for induction of mIL-6 in the M1 cell line. The ED50 in this assay is 0.75-0.4ng/mL, which corresponds to a specific activity of 1.3 x 106 - 2.5 x 106 Units/mg. When used at 10ng/mL, this recombinant protein has also been found to support mouse embryonic stem cells in an undifferentiated state at same concentration.
Unlabeled
E. coli
0.1mg/mL
Liquid
16878
Mouse LIF
Affinity chromatography
E. coli
RUO
Recombinant
Mouse LIF
Control, Functional Assay
Mouse
Ms
Recombinant
Safety and Handling

Safety and Handling

ShelfLife : 12 Months

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