Invitrogen™ MCP-5 Mouse Instant ELISA™ Kit

Instant ELISA Kit

Supplier:  Invitrogen™ BMS6007INST

Catalog No. 50-182-75

Includes: Aluminum pouch(es) with a Microwell Plate coated with Polyclonal Antibody to mouse MCP-5, Biotin-Conjugate (anti-mouse MCP-5 polyclonal antibody), Streptavidin-HRP and Sample Diluent, lyophilized
Aluminum pouch(es) with a mouse MCP-5 Standard curve (coloured)
Wash Buffer Concentrate 20x (phosphate-buffered saline with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Sample Diluent (Use when an external predilution of the samples is needed)
Stop Solution (1M Phosphoric acid)
Adhesive Plate Covers

Description

The Mouse Moncyte Chemoattractant Protein 5 (Ms MCP-5) ELISA quantitates Ms MCP-5 in mouse serum, plasma, cell culture supernatants or other body fluids. The assay will exclusively recognize both natural and recombinant Ms MCP-5. Principle of the method The Mouse MCP-5 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Chemotactic factor that attracts eosinophils, monocytes, and lymphocytes but not neutrophils. Potent monocyte active chemokine that signals through CCR2. Involved in allergic inflammation and the host response to pathogens and may play a pivotal role during early stages of allergic lung inflammation.

Specifications

• Accession Number: Q62401
• Assay Sensitivity: 2.3 pg/mL
• Product Type: ELISA Kit
• Target Species: Mouse
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: MCP-5,Scya12
• Intraassay CV: 6.7%
• Label or Dye: HRP
• Regulatory Status: RUO
• Storage Requirements: -20°C
• Target: Mouse
• Total Assay Time: 3 hr. 10 min.

• Assay Range: 15.6-1,000 pg/mL
• Conjugate: Biotin
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Application): ELISA
• Gene ID (Entrez): 20293
• Interassay CV: 10.7%
• Kit Contents: Pre-coated 96 well Microplate, Biotinylated Detection Antibody, SAV-HRP, Sample Diluent, lyophilized, Standard curve strips, Wash Buffer Concentrate, Substrate Solution, Stop Solution, Lysis Buffer 10x, Adhesive Plate Covers
• Quantity: 128 Tests
• Sample Volume: Plasma, 50 μL; Serum, 50 μL; Supernatant, 50 μL
• Synonym: CCL12, Monocyte chemotactic protein 5, SCYA12
• Test Time: 20 min.

For Research Use Only