Invitrogen™ MIP-1a (CCL3) Mouse Uncoated ELISA Kit

Uncoated ELISA Kit

Supplier:  Invitrogen™ 885601388

Catalog No. 50-112-5172

Includes: Capture Antibody Concentrate
Detection Antibody Concentrate
Mouse MIP-1 alpha Standard (lyophilized): 100pg/mL upon reconstitution
Coating Buffer Concentrate
Assay Buffer A Concentrate (PBS with 1% Tween 20 and 10% BSA)
pre-titrated Streptavidin-HRP
Tetramethylbenzidine (TMB) Substrate Solution
96 Well Plate included with product Cat. #'s ending in suffixes -22, -76, -86

Description

The Mouse Macrophage Inflammatory Protein 1 alpha (CCL3) (MIP-1α) Uncoated ELISA Kit contains pre-matched antibody pairs, and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of mouse MIP-1α. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

CCL3 (MIP1a, Macrophage Inflammatory Protein-1 alpha, MIP-1 alpha) has a molecular weight of approximately 8 kDa, belongs to the family of chemotactic cytokines, and is involved in chemotactic and proinflammatory effects, and homeostasis. CCL3 is produced by lymphocytes, macrophages and dendritic cells, and is involved in inflammatory response of blood monocytes and tissue macrophages. Also, CCL3 can exist as a naturally occurring heterodimer with MIP-1beta and has been shown to have antiviral activity against HSV-1. CCL3 protein can bind with high affinity to the CCR1 and CCR5 receptors.

Specifications

• Accession Number: P10855
• Assay Sensitivity: 0.8 pg/mL
• Product Type: ELISA Kit
• Target Species: Mouse
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: AI323804,G0S19-1,LD78alpha,MIP-1alpha,MIP1-(a),MIP1-alpha,Mip1a,Scya3
• Label or Dye: HRP
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Target: Mouse
• Total Assay Time: 24 hr. 30 min.

• Assay Range: 0.8-50 pg/mL
• Conjugate: Biotin
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Application): ELISA
• Gene ID (Entrez): 20302
• Kit Contents: Capture Antibody: Pre-titrated; purified antibody, Detection Antibody: Pre-titrated; biotin-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, 10X Coating Buffer: Buffer for plating the Capture Antibody, 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme, Enzyme: Pre-titrated Avidin-HRP, Substrate: 1X TMB Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: Corning Costar 9018 (included with product Cat. Nos. ending in suffixes -22; -76; -86)
• Quantity: 10 x 96 Tests
• Sample Volume: Plasma, 50 μL; Serum, 50 μL; Supernatant, 50 μL
• Synonym: Small inducible cytokine A3, SCYA3, CCL3
• Test Time: 1 hr. 45 min.