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Invitrogen™ MIP-3 alpha (CCL20) Mouse ELISA Kit
Sandwich ELISA Kit
Supplier: Invitrogen™ EMCCL20
Includes: Pre-coated plate(s), standard, detector antibody, HRP conjugate, diluents, wash buffer, chromogen, stop solution, and plate covers.
Description
The Mouse Macrophage Inflammatory Protein 3 alpha (CCL20) (Ms MIP-3α) ELISA quantitates Ms MIP-3α in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms MIP-3α. Principle of the method The Mouse MIP-3α solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Macrophage Inflammatory Protein-3 alpha (MIP-3 alpha), also called CCL20, is expressed in the liver, lungs, lymph nodes and peripheral blood lymphocytes. MIP-3 alpha is strongly up regulated by inflammatory signals, and down regulated by the anti-inflammatory cytokine IL-10. MIP3alpha signals through the G protein-coupled receptor, CCR6, and acts as a chemoattractant to lymphocytes and dendritic cells.Specifications
O89093 | |
3 pg/mL | |
Solid-phase sandwich ELISA | |
ELISA Kit | |
Plasma, Serum, Supernatant | |
96 assays | |
ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis | |
20297 | |
<12% | |
Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers | |
96 Tests | |
Inflammation | |
2°C to 8°C | |
Mouse | |
4 hr. 45 min. |
2.06-1500 pg/mL | |
3pg/mL to 1500pg/mL | |
Biotin | |
Cytokines & Receptors | |
This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, CD30L, CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL- 4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF. | |
Mouse | |
Colorimetric Microplate Reader | |
Ccl20 | |
<10% | |
HRP | |
RUO | |
Plasma, 50 μL; Serum, 50 μL; Supernatant, 100 μL | |
CKb4, LARC, MIP-3A, MIP-3[a], MIP3A, ST38, Scya20, exodus-1 | |
1 hr. 20 min. |
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