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Invitrogen™ TNF alpha Mouse ELISA Kit, High Sensitivity Non-distribution product as customer accommodation.

High Sensitivity Sandwich ELISA Kit

Manufacturer:  Invitrogen™ BMS607HS

Catalog No. 50-112-9007


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Includes: Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to mouse TNF-alpha
Biotin-Conjugate anti-mouse TNF-alpha monoclonal antibody
Streptavidin-HRP
Mouse TNF-alpha Standard lyophilized, 200pg/mL upon reconstitution
Calibrator Diluent
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Amplification Diluent Concentrate (2x)
Amplification Reagent I
Amplification Reagent II
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Adhesive Films

Description

Description

The Mouse High Sensitivity Tumor Necrosis Factor alpha (HS TNFα) ELISA quantitates Ms HS TNFα in mouse serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms HS TNFα. Principle of the method The Mouse HS TNFα solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A special amplification reagent step is added to achieve the high sensitivity capability. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Tumor Necrosis Factor is a polypeptide cytokine produced by monocytes and macrophages. It functions as a multipotent modulator of immune response and further acts as a potent pyrogen. TNF-alpha circulates throughout the body responding to stimuli (infectious agents or tissue injury), activating neutrophils, altering the properties of vascular endothelial cells, regulating metabolic activities of other tissues, as well as exhibiting tumoricidal activity by inducing localized blood clotting. TNF-alpha also inhibits lipoprotein lipase activity resulting in cachexia, a physical wasting condition. Activation of B-cells by the Epstein Barr virus can be inhibited by TNF-alpha. Due to its varied actions throughout the immune system, TNF-alpha may play a role in the pathogenesis of many disease states. TNF-alpha production is mediated by the action of lymphokines and endotoxins on the macrophage. Purified monocytes produce TNF-alpha within four hours of stimulation by recombinant IL-2 and there is some in vitro evidence to suggest that TNF-alpha is expressed at high levels and with prolonged kinetics in T cells stimulated by both CD2 and CD28. Secretion of TNF-alpha is enhanced by gamma interferon. TNF then induces or enhances the specific production of Class I MHC antigen, GM-CSF, and IL-1.
Specifications

Specifications

P06804
Biotin
21926
Adhesive Plate Covers
96 tests
0.75 pg/mL
50μL
Mouse
1 hr 20 min
Mouse
Cachectin, Tumor necrosis factor alpha, DIF
9.2%
3.13-200 pg/mL
ELISA
Colorimetric Microplate Reader
ELISA Kit
RUO
Plasma,Serum,Supernatant
Plasma,50 μL|Serum,50 μL|Supernatant,50 μL
2-8°C
4 hr
Tnf
4.7%
HRP
Documents
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