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Mouse Thrombopoietin (TPO) Carrier-Free, Recombinant Protein, eBioscience™ Non-distribution product as customer accommodation.

Recombinant Protein

Manufacturer:  Invitrogen 34868663

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Catalog No. 50-112-3514


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Description

Description

Thrombopoietin (TPO, THPO, MPL ligand, megakaryocyte colony-stimulating factor) is responsible for the generation of platelets through its action on hematopoietic cells committed to the megakaryocyte lineage. Binding of thrombopoietin to its receptor, MPL (CD110), initiates a signaling cascade via JAK/STAT, MAPK, and PI3K/AKT signaling pathways that leads to the proliferation and differentiation of megakaryocytes and, ultimately, to the production of platelets (thrombopoiesis). Thrombopoietin is constitutively produced by the liver and has a predicted molecular weight of 38 kDa. Description: Thrombopoietin (TPO, THPO, MPL ligand, megakaryocyte colony-stimulating factor) is responsible for the generation of platelets through its action on hematopoietic cells committed to the megakaryocyte lineage. Binding of thrombopoietin to its receptor, MPL (CD110), initiates a signaling cascade via JAK/STAT, MAPK, and PI3K/AKT signaling pathways that leads to the proliferation and differentiation of megakaryocytes and, ultimately, to the production of platelets (thrombopoiesis). Thrombopoietin is constitutively produced by the liver and has a predicted molecular weight of 38 kDa. Applications Reported: Recombinant human TPO is biologically active and can promote proliferation of human MO7e cells in culture. Applications Tested: The ED50 of this protein, as measured by proliferation of human MO7e cells, is less than or equal to 1.0ng/mL, which corresponds to a specific activity of at least 1.0 x 10e6 Units/mg. Source: E. coli (amino acids Glu22-Thr195; accession # NP_001166976.1). Bioactivity: The ED50 of this protein, as measured by proliferation of human MO7e cells, is less than or equal to 1.0ng/mL, which corresponds to a specific activity of at least 1.0 x 10e6 Units/mg. Endotoxin: Less than 0.1ng/ug cytokine as determined by the LAL assay. Purity: >98% as determined by SDS-PAGE and HPLC. Molecular Weight: 18.7 kDa. Storage and handling: For best recovery, quick spin vial prior to opening. Use in sterile environment. Reconstitute in water too a concentration of less than or equal to 0.5mg/mL. This protein dissolves slowly. Do not vortex. Store lyophilized protein at less than or equal to -20°C. Reconstituted protein solution can be stored with carrier-protein (e.g., 0.1% BSA) at less than or equal to -20°C.
Specifications

Specifications

P40226
Lyophilized
21832
Mouse Thrombopoietin (TPO) Carrier-Free
SDS-PAGE and HPLC
10μg
-20°C
E. coli (amino acids Glu22-Thr195; Accession # NP_001166976.1)
Less than 0.1ng/μg cytokine as determined by the LAL assay
Thpo
The ED50 of this protein, as measured by proliferation of human MO7e cells, is less than or equal to 1.0ng/mL, which corresponds to a specific activity of at least 1.0 x 106 Units/mg.
Mouse
Ms
Recombinant
Control, Functional Assay
0.01M tris with no preservative; pH 8.0
18.7
8.0
Affinity chromatography
E. coli
RUO
Recombinant
Mouse Thrombopoietin (TPO) Carrier-Free
Control, Functional Assay
Recombinant Protein, Carrier-Free
Unlabeled
E. coli
Safety and Handling

Safety and Handling

ShelfLife : 12 Months

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